Journal: Experimental Neurobiology

Article Title: LGR5 and Downstream Intracellular Signaling Proteins Play Critical Roles in the Cell Proliferation of Neuroblastoma, Meningioma and Pituitary Adenoma

doi: 10.5607/en.2019.28.5.628

Figure Lengend Snippet: Patient description and tumor sample characteristics

Article Snippet: Human brain whole tissue lysates and brain tissue slides from normal adults were obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Concentration Assay

Journal: Experimental Neurobiology

Article Title: LGR5 and Downstream Intracellular Signaling Proteins Play Critical Roles in the Cell Proliferation of Neuroblastoma, Meningioma and Pituitary Adenoma

doi: 10.5607/en.2019.28.5.628

Figure Lengend Snippet: Expression of LGR5, hnRNPA2B1, and hnRNPH3 proteins in human normal, benign meningioma, and pituitary adenoma tissues, and immunohistochemistry for LGR5 and hnRNPH3 in meningioma and pituitary adenoma tissues with immunofluorescence confocal microscopy. (A) Comparison of expression levels of LGR5, hnRNPA2B1, and hnRNPH3 between normal, benign meningioma, and pituitary adenoma by western blotting. (B) Confocal images of the human normal brain (top), meningioma (middle) and pituitary adenoma (bottom) tissues stained with antibodies against LGR5 (green) and hnRNPH3 (red). Scale bar: 20 μm. The number of LGR5 + /hnRNPH3 + cells per DAPI positive cells and hnRNPH3 + cells per LGR5 positive cells was counted and expressed as %.

Article Snippet: Human brain whole tissue lysates and brain tissue slides from normal adults were obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Confocal Microscopy, Comparison, Western Blot, Staining

Journal:

Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract

doi: 10.1128/IAI.72.6.3171-3178.2004

Figure Lengend Snippet: AOAH expression in murine tissues. (A) Northern analysis of multiple tissue RNAs from C57BL/6 mice (Clontech) with labeled AOAH cDNA as probe (top). The blot was stripped and reprobed to detect β-actin (bottom). The migration positions of size markers (in kilobase pairs) are shown. (B) AOAH activity in lysates of freshly harvested C57BL/6 mouse tissues. Measurements were performed in duplicate. The bars show means plus one standard deviation of data from three independent experiments.

Article Snippet: A mouse multiple tissue northern blot (BD Biosciences Clontech) was probed with a 32 P-radiolabeled 1-kb (Asp718 to HindIII) cDNA fragment of the 5′ coding region of mouse AOAH cDNA (GenBank no. {"type":"entrez-nucleotide","attrs":{"text":"AF018172","term_id":"2529570","term_text":"AF018172"}} AF018172 ).

Techniques: Expressing, Northern Blot, Labeling, Migration, Activity Assay, Standard Deviation

Journal: iScience

Article Title: Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout

doi: 10.1016/j.isci.2023.107448

Figure Lengend Snippet:

Article Snippet: Human corpus callosum Frozen tissue (Multiple sclerosis adult) , BioChain , T12346045Msc.

Techniques: Recombinant, In Situ, Transfection, Retroviral, Transgenic Assay, Plasmid Preparation, Sequencing, Homologous Recombination, shRNA, Control, Luciferase, Software